Calibrate a probe and refine R²
DiffAtOnce Legacy · desktop

00:00 — Start an independent probe study
Some published desktop labels remain in Spanish; this English narration guides the controls. We reopen a copy of the synthetic project and go directly to Probes before selecting an active series. This independent study recognizes all three ramps. Review the proposed reference and its conditions.

00:20 — Monitor the local reference
Choose peak opens the spectrum. A double click centers the window on the synthetic signal's local maximum. Check the region and possible overlaps before using it as a reference.

00:33 — Compare every slope
Calculate all slopes displays the three ramps with separate timings and fits. This comparison does not yet require a reference diffusion coefficient. The calibrated gradient has not been determined.

00:47 — Inspect the original line
The first ramp contains a deliberately perturbed observation. Refine R² retains a minimum point count and gradient range. The search is bounded and does not guarantee reaching its target.

01:01 — Review before applying
The proposal compares the current fit with a twenty-two-point selection. It suggests excluding one observation without changing intensities. Review that exclusion: the current selection remains unchanged until you apply it.

01:16 — Apply an explicit selection
Apply selection updates this ramp's fit and enables undo. A perfect R² in synthetic data does not physically validate the probe. Review the reference, medium and temperature before deriving a calibration.

01:31 — Restore the previous selection
Undo refinement restores all twenty-three points and the original fit. This walkthrough demonstrates a reversible selection. We have not saved a physical calibration or sent a synthetic probe to the cloud.

Synthetic data demonstration. This is not experimental validation or a confirmed molecular identification.