WEBVTT

00:00:00.400 --> 00:00:05.421
Double-clicking the spectrum locates the nearby maximum and opens its attenuation.

00:00:05.421 --> 00:00:08.617
Check the marked position and extraction half-width.

00:00:08.617 --> 00:00:15.920
The same region is used throughout the ramp; the maximum is not chased in each spectrum.

00:00:15.920 --> 00:00:20.942
Here we use a project copy without changing the saved map.

00:00:22.942 --> 00:00:31.138
The D-only option fits monoexponential attenuation and reports measured diffusion, final diffusion and fit statistics.

00:00:31.138 --> 00:00:35.509
Check the internal reference and temperature normalization used.

00:00:35.509 --> 00:00:40.427
A high coefficient of determination does not demonstrate monodispersity.

00:00:40.427 --> 00:00:46.983
This fit alone does not produce a distribution or identify a compound.

00:00:48.983 --> 00:00:54.516
Switch to the linear view to inspect log normalized intensity against b.

00:00:54.516 --> 00:00:58.665
Review valid and excluded observations, the fit and residuals.

00:00:58.665 --> 00:01:02.815
Do not remove observations only to improve R squared.

00:01:02.815 --> 00:01:10.192
Any manual or automatic exclusion must retain a visible criterion and the observations no longer used.

00:01:12.192 --> 00:01:18.713
Calculate distribution inverts this region's attenuation using the selected method and grid.

00:01:18.713 --> 00:01:23.604
Review convergence, residuals, boundaries and moments before accepting it.

00:01:23.604 --> 00:01:30.125
Hydrodynamic radius requires documented viscosity and temperature; mass requires a compatible calibration.

00:01:30.125 --> 00:01:37.733
Adding a result is a separate action and is not performed in this video.