WEBVTT

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In internal references, review candidates and confirm the standard actually added.

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Check its region, attenuation, hydrodynamic radius and provenance.

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Detecting a signal does not automatically activate a reference.

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If you work without an internal standard, the result must retain that warning.

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This walkthrough uses demonstration settings, not a validated calibration.

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In molecular-weight analysis, review the inversion engine and mass model.

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Changing engine creates a different protocol; it does not rewrite historical results.

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Linear-polymer calibrations do not validate absolute masses of stars or brushes.

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Distinguish measured diffusion, corrected diffusion and apparent mass before comparing samples.

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Open advanced mode only when you need to inspect the grid, regularization and stopping criteria.

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Standard values support a reproducible workflow but do not guarantee convergence or identifiability.

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Do not relax a check merely to obtain an attractive figure.

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Retain the method, parameters, warnings and calculation status in the revision you accept.

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Review probe, calibration, solvent, viscosity and temperature.

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In the principal KK2 protocol, the TTMS reference factor and thermal factor are retained per block and applied together exactly once to the complete axis.

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Do not replace the experimental TBO calibration with a nominal gradient.

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This video shows where to inspect conditions without recalculating original data.