1. Install the correct edition
Download the Legacy Original 1.0.0 MSI or install through Studio 0.1.3 or later. Check name, version, size and SHA-256. It installs per user and requires Windows x64 and .NET Framework 4.8. Legacy WPF is a separate product with its own installation.
2. Recover the working folder
Choose your experiment location in the working-folder bar. The preference is stored locally for the computer. Close and reopen to check the recovered location. That folder is not included in the public MSI.
3. Open Bruker spectra
Select the folder and processed experiment. Check the acquisition and the corresponding 1r/procs files. Review ramp acquisition count and order; point count does not replace checking gradients or timings.
4. Select resonances
Enable resonance selection and choose frequencies on the plot. Check that each marker corresponds to the intended peak, then review the signals before proceeding to attenuation and diffusion.
5. Inspect point fitting
Compare the table with the plotted points and fitted line. Inspect slope, coefficients and R², and retain the reason for every exclusion. Non-positive intensities, non-finite values or insufficient selection require review. Residual comparison helps detect problems but does not alone establish an experimental exclusion.
6. Check diffusion
Before interpreting D, inspect nucleus, sequence, calibration and effective timings. Check temperature and viscosity units. Results depend on these conditions as well as the slope.
7. Shape and size
Choose sphere, cylinder or ellipsoid and review geometry parameters. Check that dimensions, radii and plots refer to the same signal and conditions. One diffusion coefficient does not determine a unique molecular shape.
8. Wirtz and Chen
Select the method and inspect solvent radius, factor and convergence state. The iteration plot should end at the displayed radius. Convergence establishes numerical solution of the model, not experimental validity of its assumptions.
9. Save and reopen
Save the analysis and reopen it through Catalogs → Open complete analysis. Compare included and excluded points, fits, conditions and results. Use a separately named copy to try another selection, preserving the original. Back up local data before updating.
10. Equations and contextual help
Use F1 from Fitting, Diffusion, Shape and size, or Wirtz and Chen to open the corresponding topic. View equation shows the screen’s model. Review its variables and units alongside the effective analysis parameters.
11. Catalogs and saved data
Catalogs and analyses use local SQLite. Check the selected record before editing and preserve its units. Invalid historical records need diagnosis; an unknown result is not replaced with zero. The public MSI does not carry your projects or spectra.
12. Update with provenance
Save changes and close Legacy Original. Studio 0.1.3 uses the compatible stable-v3 channel and verifies the package before installation; earlier clients retain their channels. After updating, inspect the launched version and a copy of a saved analysis. Updating is neither a new calculation nor calibration confirmation.